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| Checkpoint : Nucleotide Spindle Checkpoint |
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| S.
cerevisiae |
S. pombe |
Human |
Structure
and Function |
Description |
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| MAD1 |
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coiled-coil |
kinase
substrate, interacts with Mad2 and Cdc20 |
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| MAD2 |
mad2
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MAD2
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prenyltransferase aipha subunit |
kinetochore,
interacts with Mad1 and Cdc20 |
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| MAD3 |
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interacts with
Cdc20 |
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| BUB1 |
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BUB1 (2q12-13) |
protein kinase |
kinetochore,
phosphorylate Bub3 |
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BUBR1
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protein kinase |
kinetochore,
interacts with Bub1 |
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| BUB2 |
cdc16
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GAP
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| BUB3 |
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BUB3
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WD repeat, 37kD |
kinetochore,
interacts with Bub1 |
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| MPS1(monopolar spindle) |
mph
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TTK?
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protein kinase |
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| CDC20 |
Slp1
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WD-repeat |
inhibited by
interaction with Mad1, 2 and 3 |
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| Hct1/Cdh1 |
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WD-repeat |
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Nucleotide
Spindle Checkpoint
Signal in S. cerevisiae |
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| MPS1 |
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| ↓ |
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| Bub1, Bub3, Mad2 |
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| ↓ |
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| Mad1 |
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| ↓ |
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| Bub2, Mad3 |
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| ↓ |
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| Mitotic arrest |
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